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The column was equilibrated with lysis buffer, the supernatant was loaded, the column was washed three times with washing buffer (50 mM NaH 2 PO 4 /Na 2 HPO 4 , 0.3 M NaCl, 40 mM imidazole, pH 8.0), and the recombinant proteins were eluted with elution buffer (50 mM Na 2 HPO 4 /Na 2 HPO 4 , 0.3 M NaCl, 250 mM imidazole, pH 8.0)

The statute provides that the full reward must be available to all similarly situated individuals, and that all such individuals must be given the opportunity to qualify for the reward at least once each year

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A splash and a flounder proclaimed that Lord Peter had walked, like Johnny Head-in-Air over the edge of the impluvium, papers and all